The new generation drug candidate molecules: Spectral, electrochemical, DNA-binding and anticancer activity properties

Gölcü A., Muslu H., Kiliçaslan D., Çeşme M., Eren Ö., Ataş F., ...More

Journal of Molecular Structure, vol.1119, pp.96-109, 2016 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 1119
  • Publication Date: 2016
  • Doi Number: 10.1016/j.molstruc.2016.03.078
  • Journal Name: Journal of Molecular Structure
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.96-109
  • Keywords: 5-Fluoracil, DNA binding, Electroanalysis, Metal based drugs, xCELLigence
  • Ondokuz Mayıs University Affiliated: No


The new generation drug candidate molecules [Cu(5-Fu)2Cl2H2O] (NGDCM1) and [Zn(5-Fu)2(CH3COO)2] (NGDCM2) were obtained from the reaction of copper(II) and zinc(II) salts with the anticancer drug 5-fluoracil (5-Fu). These compounds have been characterized by spectroscopic and analytical techniques. Thermal behavior of the compounds were also investigated. The electrochemical properties of the compounds have been investigated by cyclic voltammetry (CV) using glassy carbon electrode. The biological activity of the NGDCM1 and NGDCM2 has been evaluated by examining their ability to bind to fish sperm double strand DNA (FSdsDNA) with UV spectroscopy. UV studies of the interaction of the 5-Fu and metal derivatives with FSdsDNA have shown that these compounds can bind to FSdsDNA. The binding constants of the compounds with FSdsDNA have also been calculated. Thermal decomposition of the compounds lead to the formation of CuO and ZnO as final products. The effect of proliferation 5-Fu, NGDCM1 and NGDCM2 were examined on the HeLa cells using real-time cell analyzer with three different concentrations.