Akademik Veri Yönetim Sistemi
Caucasian Journal of Science, cilt.2023, sa.10, ss.38-48, 2023 (Hakemli Dergi)
RORA (RAR-Related Orphan Receptor Alpha) regulates estrogen synthesis by
transcribing steroid related genes. Litter size is an important reproductive trait
associated with estrogen synthesis. it is critical to investigate the relationship between
the RORA gene and sheep litter size. In this study, a 23 base pair nucleotide sequence
mutation in a gene called RORA and the effect of this mutation on the productivity of
sheep offspring were investigated. In this study, a 23 bp nucleotide sequence mutation
was found in intron 1 of the RORA gene in 35 female Bafra ewes. Ten ml of milk was
collected from each of the 35 samples. The litter size of the DD genotype was
significantly higher than the ID genotype and the II genotype in the second born litter
size (p> 0.05). The relationship between combined genotypes and average litter size
showed that ewes with homozygous (DD) genotype had larger lambs than ewes with
heterozygous (ID) genotype. Multiple pregnancy rates in the first and second litters
were higher than the average stillbirth rate (p> 0.05). To summarize, this study provided
theoretical references for further research on the function of RORA gene and breeding
of Bafra sheep. The 23-bp indel variants can be used as molecular markers for the size
of first and second born offspring of ewes in MAS (marker assisted selection) breeding.