Akademik Veri Yönetim Sistemi
5th INTERNATIONAL “ARTEMIS” CONGRESSES ON LIFE, ENGINEERING, AND APPLIED SCIENCES, 1 - 03 Ekim 2023, ss.138-139, (Özet Bildiri)
Chitinase is an enzyme that breaks down chitin, a complex carbohydrate found in the cell walls
of fungi, exoskeletons of arthropods (such as insects and crustaceans), and some other organisms.
Chitinase was extracellularly produced from Jiangella alba in ISP2 liquid medium (4 g/L yeast extract,
10 g/L malt extract, and 4 g/L glucose, pH 7.0 ± 0.2) supplemented with colloidal chitin (10 g/L) for
7 days at 32 °C (Ding vd., 2023; Adıgüzel vd., 2023). The enzyme was retrieved from the culture by
centrifugation (10.000 x g, 10 min) and then subjected to ammonium sulfate precipitation with 80%
saturation. Precipitated enzyme dissolved in 50 mM phosphate buffer (pH 7.0) was used as crude
chitinase. Enzyme activity was assayed by the dinitrosalicylic acid (DNS) method using colloidal chitin
as substrate (Yahiaoui 2019). Crude chitinase was active at a temperature range from 20 to 50 °C and
exhibited maximal activity at 45 °C. Its maximum activity was recorded in 50 mM phosphate buffer at
pH 6.0. The enzyme lost its activity about 28, 43, and 50% after pre-incubated at 40, 45, and 50 °C for an
hour. Maximum chitinase activity was observed in the presence of NaCl. Elevated concentration of NaCl
caused a gradual decrease in the chitinase activity. Adding Mg2+ and Ca2+ at a five mM final concentration
to the reaction mixture increased the activity of crude chitinase. On the other hand, a significant decrease
in the enzyme activity was observed when the Cu2+ was introduced to the reaction mixture. In addition,
5 mM Triton X-100 slightly increased the activity of chitinase. Results showed that the chitinase from
Jiangella alba has a potential for use in various industrial applications such as biological treatment of
seafood, textile processing, drug development, and material design for packaging. In addition, it may
be used as a biocontrol agent.