E-Selectin expression in the mouse testis after experimental testicular torsion (ischemia/reperfusion)

Selçuk M., Celebi M., Gülbahar M. Y., Kabak Y. B., Çevik M.

VETERINARSKI ARHIV, vol.84, no.2, pp.167-176, 2014 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 84 Issue: 2
  • Publication Date: 2014
  • Journal Name: VETERINARSKI ARHIV
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.167-176
  • Keywords: E-selectin, ischemia, reperfusion, mouse, testis, GERM-CELL APOPTOSIS, ISCHEMIA-REPERFUSION, RECRUITMENT, NEUTROPHILS, ACTIVATION, PATHWAY, KINASE, INJURY, REPAIR
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

Germ cell-specific apoptosis occurs after ischemia/reperfusion of the testis and is dependent on E-selectin expression. The aim of the study was to determine differences in E-selectin expression in testes tissues of control, sham and treatment groups after ischemia/reperfusion in mice. Mice were subjected to 720 degrees testicular torsion for 1 h or 2 h duration (ischemia) followed by detorsion (reperfusion). After 2 h of reperfusion, the testes were fixed in Bouin fixative and immunohistochemical analysis performed for E-Selectin expression. E-selectin expression increased in the ischemic testis and contralateral testis after 2 h of reperfusion in mice. This increase in E-selectin expression may confirm that E-selectins play a key role in mediating of apoptosis in germ cells after ischemia/reperfusion. Thus, the blockage of E-selectins might be a strategy for rescue of post-ischemic testes.