Rice is a staple crop grown in the world especially in the Asian region. The rice faces with differentbiotics and abiotic stress factors, especially drought stress. Turkish upland rice is one of the special ricetypes grown on drought stress condition, having a large potential value. Our aim was to analyse theeffects of different concentrations of BAP+NAA and KIN+2,4-D combination on rice grown in vitrocondition. For this purpose, mature seeds of Turkish upland rice were selected as starting material fordirect/indirect regeneration response in serial treatment. The best medium for induction of shootregeneration was MS 1 supplemented with 1.5 mg L -1 BAP and 0.5 NAA mg L -1 . The results showedthat the maximum callus formation (65.54 %) was obtained on MS medium containing 2.4-D (2.0 mg L -1 ) within 21 days from nodes. With this study, we have developed a plant tissue culture for Turkishupland rice, suggesting positive methods for rice biotechnology.