Molecular identifications of rhizobial samples isolated from Phaseolus vulgaris L. in Eskişehir province of Turkey


Biological Diversity and Conservation, vol.11, no.2, pp.106-114, 2018 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 2
  • Publication Date: 2018
  • Journal Name: Biological Diversity and Conservation
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.106-114
  • Ondokuz Mayıs University Affiliated: Yes


In this study three rhizobial samples (R28, R51, R66) were anaysed using molecular phylogenetic methods.Rhizobial samples were isolated (from P. vulgaris L. root nodules collected from Eskişehir province of Turkey) andmorphologically characterized in a previous study. Analyses were performed using a concatenated data set whichcomposed of three housekeeping genes (recA, atpD and glnII). Additionally two symbiotic genes, nodA and nifH, weresequenced and analysed to determine the symbiotic plasmid type. As a result of housekeeping gene phylogeny, isolateR28 was found to be related to R. laguerreae. Although other two isolates, R51 and R66, placed in the same lineagewith R. sophoriradicis, they showed enough divergence to be considered as a new species. But this presumption need tobe confirmed with further investigations. Network analyses of nodA and nifH genes clearly showed that R28 has aunique symbiotic plasmid. On the other hand, symbiotic plasmids of R51 and R66 found to be related to p42d which isthe most common symbiotic plasmid in rhizobia nodulating P. vulgaris L. in Europe and also Turkey. In this study firstreport of R. laguerreae from Turkey is presented. More over, first report of a R. laguerreae isolate from P. vulgaris L.root nodules is also presented and suggested isolate R28 as R. laguerreae bv. phaseoli. Additionally, molecular hints fora potentially new rhizobial species have also given. But this presumption must be supported with some additionalmolecular and morphological investigations.