Jurinea humilis DC. Polar Extract: HPLC Analysis, Photoprotective, Antioxidant Activities and Bioactive Content

Ayad R., Keskinkaya H. B., Atalar M. N., Lefahal M., Zaabat N., Makhloufi E. H., ...More

Chemistry Africa, vol.6, no.2, pp.827-836, 2023 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.1007/s42250-022-00525-y
  • Journal Name: Chemistry Africa
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
  • Page Numbers: pp.827-836
  • Keywords: Antioxidant, Jurinea humilis, Photoprotective, Polyphenols, Sustainability, Ultraviolet radiation
  • Ondokuz Mayıs University Affiliated: No


The use of botanical antioxidants to protect human skin from the harmful effects of ultraviolet radiation (UVR) opens the door to new and exciting avenues of research in photoprotection. Besides, for the sake of long-term sustainability, this strategy is both environmentally and human-friendly. To that end, the current study focuses on the polar extract (n-butanol) of Jurinea humilis DC. Several spectrophotometric methods were used to determine the total bioactive content of this extract. HPLC analysis was also used to determine the chemical profile of Jurinea humilis n-butanol extract. In addition, the antioxidant capacity of the sample was determined using the phosphomolybdenum method, as well as DPPH and H2O2 scavenging activities. The sun protection factor (SPF) was calculated using Mansur's dilution method, to assess photoprotective activity. The results showed that polar extract contained a high level of polyphenols, specifically phenolic content (171.18 ± 1.06 mg GAE/g extract), flavonol content (128.62 ± 4.42 mg RE/g extract), and flavonoid content (109.46 ± 0.25 mg QE/g extract), as well as potential antioxidant activity using phosphomolybdenum method (199.72 ± 3.71 mg AAE/g extract), DPPH inhibition (> 90%) and H2O2 scavenging activity (> 70%). Furthermore, at a concentration of 2 mg/mL, it has higher photoprotective properties, with a maximum SPF of 36.22 ± 0.07. Several polyphenols were discovered for the first time in the active extract using HPLC analysis. Therefore, Jurinea humilis polar extract appears to be a promising cosmetic ingredient.