Akademik Veri Yönetim Sistemi
2. Uluslararası Sağlık Bilimleri Kongresi, Van, Türkiye, 18 - 19 Aralık 2023, ss.228-231, (Tam Metin Bildiri)
Aim: Sideritisspecies belong to the Lamiaceae family and are generally known as mountain tea. These species are used for flavoring and therapeutic purposes as well as tea among the folk. The aim of the study is to determine the chemical characterization of the essential oil, volatile components of the 5% infusion and n-hexane extract, total fenolic content and lipoxygenase inhibitory activity of Sideritis libanotica subsp. microchlamys (Hand.-Mazz.) Hub.-Mor. collected from Mardin. Material and Method: The essential oil of the aerial parts of S. libanotica subsp. microchlamys was obtained by hydrodistillation using Clevenger apparatus. 5% infusion of the plant was prepared and volatile components of the infusion were extracted by Headspace-Solid Phase Microextraction (HS-SPME). Aerial parts of the plant were macerated with n-hexane. The chemical composition of the essential oil, volatile components of the 5% infusion, and n-hexane were analysed by GC and GC/MS methods. The total phenol contents of the samples were tested using the Folin-Ciocalteau method and expressed as the gallic acid equivalent (GAE). The lipoxygenase (LOX) inhibitory activity of the samples was executed spectrophotometrically using the kit procedure. Results: The % yield of the essential oil was found as trace (<0.01). Fourty-four compounds in the oil were identified. The main components of the essential oil were identified as hexadecanoic acid (8.8%), spathulenol (5.7%), and methyl hexadecanoate (5.2%). Six compounds were determined in the 5% infusion and the main volatile compounds were 1-octen-3-ol (80.2%), and β-pinene (6.0%). Fourteen compounds were detected in the n-hexane extract and the major constituent was hexacosane (5.4%). The total phenolic content of the 5% infusion was found to be 66 mg GAE/g extract, while no phenolic substance was detected in the n-hexane extract. The infusion and n-hexane extract did not have an inhibitory effect on the lipoxygenase enzyme at a concentration of 100 µg/mL. Conclusion: To the best of our knowledge, this study was the first to evaluate of the volatile components and lipoxygenase inhibitory activity of S. libanotica subsp. microchlamys. It is expected that this study will lead to further studies on the plant.