TEMPERATURE EFFECT ON BINDING AFFINITY AND STOICHIOMETRY BETWEEN SOME STEROIDS AND HUMAN SERUM ALBUMIN

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Ozdemir S., Biçer E.

JOURNAL OF THE CHILEAN CHEMICAL SOCIETY, vol.61, no.1, pp.2809-2815, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.4067/s0717-97072016000100013
  • Journal Name: JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2809-2815
  • Keywords: steroids, human serum albumin, binding affinity, temperature effect, voltammetry, DRUG-BINDING, DNA INTERACTION, CRYSTAL-STRUCTURE, PLASMA PROTEINS, FATTY-ACIDS, BOVINE, TESTOSTERONE, ELECTROCHEMISTRY, PROGESTERONE, FLUORESCENCE
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

The temperature dependence of the interactions between some steroids (fusidic acid, ouabain and ethynodioldiacetate) and human serum albumin (HSA) at physiological pH (7.4) were studied systematically by square-wave voltammetry. Also, these interactions were proven by means of UV-Vis. absorption spectroscopy and FT-IR spectroscopy techniques. The interaction parameters (binding constants and stoichiometries) at temperature range of 297.5-312.5 K were determined from electrochemical data. It is worthy that the temperature has played a positive role on the interactions of fusidic acid with HSA; however it has negative effective on the interaction parameters of HSA with ouabain or ethynodioldiacetate.