Interaction between Janus Green B and bovine serum albumin: Electrochemistry and spectroscopy studies

Ozdemir N., Ozdemir S., Biçer E.

CHINESE CHEMICAL LETTERS, cilt.22, sa.6, ss.717-720, 2011 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 6
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1015/j.cclet.2010.12.007
  • Dergi Adı: CHINESE CHEMICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.717-720
  • Anahtar Kelimeler: Voltammetry, Bovine serum albumin, Janus Green B, Interaction, METHYLENE-BLUE, PROTEINS, SUBSTANCES, DNA
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

In this study, voltammetric and spectroscopic investigation of the interaction between Janus Green B (JGB) and bovine serum albumin (BSA) was reported. The interaction was observed at Britton-Robinson buffer (pH 7.0). When JGB was added to solution containing BSA, the peak currents of BSA decrease with the increasing ofJGB concentrations which is due to the interaction of JGB and BSA. The binding constant ofJGB with BSA was obtained by voltammetric data. Also, this interaction was supported by means of UV-vis spectroscopic measurements. The UV vis absorption spectra of JGB in the presence of BSA decrease with the increasing of BSA concentrations. (C) 2010 Ender Bicer. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.