Assessment of ischaemia-reperfusion injury in the mice testis by using contrast ultrasound molecular imaging

Celebi M., Paul A. G. A.

ANDROLOGIA, vol.48, no.9, pp.907-913, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 9
  • Publication Date: 2016
  • Doi Number: 10.1111/and.12531
  • Journal Name: ANDROLOGIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.907-913
  • Keywords: E-selectin, microbubbles, testis, torsion, ultrasound, CELL-SPECIFIC APOPTOSIS, E-SELECTIN EXPRESSION, TESTICULAR ISCHEMIA, ISCHEMIA/REPERFUSION INJURY, NEUTROPHIL RECRUITMENT, SHEAR-FLOW, DAMAGE, MICROBUBBLES, INFLAMMATION, CYTOKINES
  • Ondokuz Mayıs University Affiliated: No

Abstract

Timely diagnosis of ischaemia-reperfusion (IR)-induced injury after testicular torsion may be critical for saving reproductive function. The purpose of this study was to detect IR-induced injury, indicated by E-selectin overexpression, in murine testis using ultrasound molecular contrast imaging. Mice underwent 720(degrees) unilateral testicular torsion (ischaemia) followed by detorsion (reperfusion), and the control group (Sham-IR) was operated identically without extended ischaemia. In a separate positive control group, TNF- was injected intratesticularly to induce inflammation and compared to intratesticular saline injection. Selectin-targeted or nontargeted ultrasound contrast microbubbles were injected intravenously, and two-dimensional (2D) real-time high-resolution ultrasound testicular imaging was performed after reperfusion or after TNF- injection. Contrast intensity levels were significantly higher in the testis of the IR group as compared to the Sham-IR group after injection of targeted contrast microbubbles. Contrast intensities were similar between the IR and Sham-IR groups after injection of nontargeted microbubbles. In addition, targeted contrast intensity levels were significantly higher in the TNF--treated group as compared to the control group. This study indicates that ultrasound contrast molecular imaging with microbubbles targeted to E-selectin can be used to assess IR-induced testicular injury.