The main purpose of this project is establishing the mother blocks with 22 clones of eight grapevine cultivars ('Sultani', 'Kalecik Karasi', 'Cabernet Sauvignon', 'Merlot', 'Shiraz', 'Mourvedrè', 'Chardonnay', 'Sauvignon Blanc'), and five clones of five rootstocks ('110 R', '1103 P', '140 Ru', 'Kober 5 BB', '41 B'), which are widely used in different viticultural areas of Turkey. Plant material was tested for eight grape viruses (GFLV, GLRaV- 1, 2, 3, 6, GVA, ArMV, GFkV, SLRSV, RpRSV, ToRSV-chi) using DAS-ELISA. Virus-infected clones were in vitro meristem cultured and subcultured to eliminate the viruses in MS media and re-tested for the infectious viruses. All previously infected clones were found to be free after in vitro meristem culture. Finally, all clones were mass-propagated by in vitro shoot-tip culture and acclimatized under mist, then transplanted into the plastic containers as a single-node cutting source of the basic nursery stocks.