A novel actinobacteria, designated strain K413(T), was isolated from soil collected from Karpaz National Park, Magusa, Northern Cyprus, and characterized to determine its taxonomic position. The isolate was found to have chemical and morphological properties associated with members of the genus Streptomyces. Phylogenetic analyses based on almost-complete 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus Streptomyces, and was shown to form a distinct phyletic line in the Streptomyces phylogenetic tree. Strain K413(T) was most closely related to Streptomyces marinus DSM 41968(T) (98.01 %). Sequence similarities with other strains of the genus Streptomyces were below 98.0%. The cell wall of the novel strain contained LL-diaminopimelic acid. The predominant menaquinone was MK-9(H-8) (45.0%). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylinositol. The major fatty acids were anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0. Based on 16S rRNA gene sequence analysis, DNA DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain K413(T). represents a novel species of the genus Streptomyces, for which the name Streptomyces karpasiensis sp. nov. is proposed. The type strain is K413(T) (=KCTC 29096(T)=DSM 42068(T)).