Determination of the Anti-methanogenic Effect of Turpentine (Pistacia terebinthus L.) Tree Leaf Extract Addition to Dry Meadow Grass by In Vitro Gas Production Method


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Bakır T., Selçuk B., Başer A., Kaya A.

4th International Conference on Advanced Engineering Technologies, Bayburt, Türkiye, 28 - 30 Eylül 2022, cilt.4, ss.689-692, (Tam Metin Bildiri)

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 4
  • Basıldığı Şehir: Bayburt
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.689-692
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The aim of this study was to determine the effect of the extract obtained from the leaves of turpentine tree (Pistacia terebinthus L.) added to dry meadow grass on gas and methane production in ruminants. Turpentine tree (Pistacia terebinthus L.) leaves were dried to extract the extracts after being collected from the field. After drying, 5 g of the ground tree leaves were taken and mixed in 80 ml of 10% methanolethanol solution for 72 hours. The obtained leaf extracts were prepared at four different doses of 0 (C), 0.6 (M1), 1.2 (M2), 1.8 (M3) ml/g DM and four replicates and the amounts of gas and methane production were determined by in vitro gas production technique. In vitro gas and methane production, methane (%), metabolic energy, net energy lactation and organic matter digestion degree were found to be significant (p<0.01). The 24-hour in vitro gas production values of the experimental groups were 42.27- 61.45 ml/200 mg DM, methane production values were 6.75- 13.12 ml, methane (%) values were 15.98- 25.23, metabolic energy values were 9.19- 11.91 MJ/kg DM and organic matter digestibility values were 64.57- 81.63 %. As a result, there is an increase in gas production as a result of fermentation due to the increase in extract in dry meadow grass. However, it did not show any antimethanogenic effect on methane production, one of the greenhouse gases originating from ruminants. For this reason, it is not recommended to prefer the use of turpentine tree (Pistacia terebinthus L.) extracts for the reduction of methane gas that causes greenhouse gas effect.