Molecular identification and phylogenetic analysis of non-cytopathic bovine viral diarrhea virus isolate obtained from a local respiratory outbreak in Northern Turkey

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Yazıcı Z., Gümüşova S., Tamer C., Cagirgan A. A., Akman A., Özan E., ...More

Medycyna Weterynaryjna, vol.73, no.5, pp.284-288, 2017 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 73 Issue: 5
  • Publication Date: 2017
  • Doi Number: 10.21521/mw.5694
  • Journal Name: Medycyna Weterynaryjna
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.284-288
  • Keywords: BVDV, ELISA, Nucleotide sequence, Phylogenetic analysis, RT-PCR
  • Ondokuz Mayıs University Affiliated: Yes

Abstract

In February 2016, a local respiratory disease outbreak with two fatalities was reported in Samsun, Turkey. A non-cytopathic bovine viral diarrhea virus (ncp-BVDV) was identified from the organ and leucocyte samples of dead juvenile heifers using RT-PCR with specific primers for the NS2/3 gene coding region. The NS2/3 gene of BVDV was sequenced and compared with other published sequences of BVDV. The sequences of our isolate which was named as Samsun TR, had 81-83% nucleotite (nt) identity for BVDV-1. Phylogenetic analysis revealed that Samsun-TR was closely related to LC089875 (Japan), AF526381 (China) and also shared 83% nucleotide(nt) identity with them. The NS2/3 gene sequence of Samsun-TR was deposited in the GenBank database with the accession number of KX428495.