Chemical constituents, in vitro antioxidant and antiproliferative activities of Perralderia coronopifolia Coss. subsp. eu-coronopifolia M. var. typica M. extract

Boussaha S., Bekhouche K., Boudjerda A., León F., Koldas S., Yaglioglu A. S., ...More

Records of Natural Products, vol.9, no.3, pp.312-322, 2015 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 3
  • Publication Date: 2015
  • Journal Name: Records of Natural Products
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.312-322
  • Keywords: Antioxidant activity, Antiproliferative activity, Asteraceae, Caffeoylquinic acids, Flavonoids, Perralderia coronopifolia, Terpenoids
  • Ondokuz Mayıs University Affiliated: No

Abstract

Phytochemical investigations of extracts from the aerial parts (leaves and flowers) of Perralderia coronopifolia Cosson resulted in the isolation of nine secondary metabolites corresponding to three flavonoids: rhamnazin(1), chrysosplenol D (3), and (2R, 3R) taxifolin (4), two monoterpene glycosides : myrtenol- β-D-glucopyranoside- 6'-O-acetate (2) and myrtenol β-D-glucopyranoside (7), a disaccharide: sucrose (9) and three di-O-caffeoylquinic acid derivatives : methyl 3, 5-di-O-caffeoyl quinate (5 ) and methyl 3,4-di-O-caffeoyl quinate (6 ) as a mixture and 1,5-di-O-caffeoylquinic acid (8). The structures were identified by spectroscopic methods such as 1H and 13C NMR, COSY, HSQC and HMBC experiments, HRESI-MS and comparison with literature data. Myrtenol-β-D-glucopyranoside-6'-O-acetate (2) was isolated in pure and native state for the first time. The other compounds are new for the genus Perralderia Cosson. The ethyl acetate extract showed a high antioxidant effect, especially DPPH radical scavenging activity with IC50=7.01±0.28µg/mL) compared to ascorbic acid ( IC50= 5±0.1µg/mL . This extract also showed antiproliferative activity against HeLa (human cervix carcinoma) and C6 (rat brain tumor) cells.