Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus in Foods of Animal Origin, Turkey

Sırıken B., YILDIRIM T., Guney A. K., Erol I., Durupinar B.

JOURNAL OF FOOD PROTECTION, vol.79, no.11, pp.1990-1994, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 79 Issue: 11
  • Publication Date: 2016
  • Doi Number: 10.4315/0362-028x.jfp-16-161
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1990-1994
  • Keywords: Beef, Fish, Methicillin-resistant Staphylococcus aureus, Milk, Pulsed-field gel electrophoresis, FIELD GEL-ELECTROPHORESIS, MULTIPLEX PCR ASSAY, SIMULTANEOUS DISCRIMINATION, BOVINE MASTITIS, MRSA, STRAINS, KOREA, MILK, VIRULENCE, WORKERS
  • Ondokuz Mayıs University Affiliated: Yes


In the present study, 175 coagulase-positive Staphylococcus (CPS) isolates recovered from samples of beef (n = 110), raw milk n 56), and fish (n = 9) were analyzed for methicillin resistance using MIC and PCR assays. Methicillin-resistant (MR) Staphylococcus aureus (SA) isolates were then characterized using pulsed-field gel electrophoresis (PFGE). According to findings, 62 (35.4%) of the isolates (44 from beef, 9 from milk, and 9 from fish) were identified as S. aureus based on the presence of the nuc gene. MRCPS was detected in 18 (10.3%) of 175 CPS isolates based on the presence of the mecA gene. Among these isolates, 15 (24.2%) were MRSA: 4 (26.7%) from beef, 2 (13.3%) from milk, and 9 (60%) from fish. However, based on the MIC assay, 21 (12.0%) of the CPS isolates (1 from beef, 15 from milk, and 5 from fish) were MRCPS, indicating a discrepancy between the results of these two methods. The PFGE results indicated genetic heterogeneity of the isolates; six PFGE clusters were found. These results confirm that MRSA is present in foods of animal origin, which is a concern to human health, and indicate the importance of method selection for determination of methicillin resistance. The identity of MR isolates should be verified by PCR to obtain more reliable results.