Prevalence and Characterization of Coagulase Positive Staphylococci Isolated from Salted Anchovy

Sırıken B., YILDIRIM T., Erol I., Durupinar B., Çiftci A., Onuk E. E.

JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, vol.22, no.4, pp.339-352, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1080/10498850.2011.651773
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.339-352
  • Keywords: coagulase positive Staphylococci, S, aureus, salted anchovy, antibiotic resistance, methicillin resistance, biofilm, PVL, genotyping, PANTON-VALENTINE LEUKOCIDIN, METHICILLIN-RESISTANT STAPHYLOCOCCI, SIMULTANEOUS DISCRIMINATION, ANTIMICROBIAL RESISTANCE, SLIME PRODUCTION, PCR ASSAY, AUREUS, STRAINS, GENES, TESTS
  • Ondokuz Mayıs University Affiliated: Yes


A total of 100 salted anchovy samples were used to investigate the prevalence of S. aureus and other coagulase positive Staphylococci (CPS) as well as to determine the methicillin (MR) and antibiotic resistance (AR) profile, the presence of Panton-Valentine leukocidine (PVL) toxin gene (lukS/F-PV), slime factor properties (SFP), and the genotypic relatedness of the isolates. Agar disc diffusion assay (ADDA) and microdilution broth susceptibility test (MDBST) were applied to compare the specificity and sensitivity of the MR detection methods. A total of 41 CPS isolates were detected at the 10(2) and 10(3) CFU/g levels in contrast to S. aureus. The 16S rRNA (genus specific) was detected in all the isolates in contrast to nuc (species-specific) and lukS/F-PV genes. A total of 16/41 isolates were found to be MR by using the three methods. Polymerase chain reaction (PCR) assay was a more sensitive and reliable method for the detection of MR isolates. The antibiotic resistance rates were 75.60, 73.17, 51.21, 31.70, 12.19, and 4.87% to penicillin, ampicillin, tetracycline, erythromycin, ciprofloxacin, and clindamycin, respectively. All the isolates were sensitive to gentamicin and vancomycin. The SFP were determined in all the isolates by using Congo Red agar, and 20 different genotypes were determined by using randomly amplified polymorphic DNA (RAPD)-PCR assay.